When culturing microbes, it is very important to provide similar environmental and nutritional conditions
that exist in its natural habitat. Hence, an artificial culture medium must provide all the
nutritional components that a bacterium gets in its natural habitat. Most often, a culture medium
contains water, a source of carbon & energy, source of nitrogen, trace elements and some growth
factors. Besides these, the pH of the medium must be set accordingly. Some of the ingredients of
culture media include water, agar, peptone, casein hydrolysate, meat extract, yeast extract and
malt extract.
Peptone-As a source of nitrogen Dextrose-As a source of energy Potato/Corn/Meat extrat/Malt Extract-As a source of Carbon Agar-As a solidifying agent
